Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards Neoatherosclerosis: A Porcine Model for Enhanced Device Testing.

Journal of cardiovascular translational research·2026
Same author

Comparative acute toxicity of intravenous paclitaxel and sirolimus in rats.

Current research in toxicology·2025
Same author

What looks like a contrast reaction may not be a contrast reaction.

European journal of radiology·2025
Same author

Ablation of symptomatic uterine fibroids with the Mirabilis system for rapid noninvasive ultrasound-guided high-intensity focused ultrasound (HIFU): a prospective observational clinical study.

La Radiologia medica·2025
Same author

Technical and clinical reasons for negative skin test results following intradermal testing of contrast media.

European journal of radiology·2025
Same author

Drug-coated Balloons in the Neurovascular Setting: A Comprehensive, Systematic Review of Current Use and Indications.

Reviews in cardiovascular medicine·2024

Related Experiment Video

Updated: Jun 13, 2026

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Pilot study on basophil activation induced by contrast medium.

Ingrid Böhm1, Ulrich Speck, Hans H Schild

  • 1Center for Molecular Imaging Research MBMB, Department of Radiology, Philipps University of Marburg, Germany. i.boehm@uni-bonn.de

Fundamental & Clinical Pharmacology
|April 24, 2010
PubMed
Summary

Contrast media (CM) injections can cause histamine release and basophil activation, indicated by CD63 expression. Individual patient factors, not the CM itself, appear to influence these hypersensitivity reactions.

More Related Videos

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
17:35

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

Related Experiment Videos

Last Updated: Jun 13, 2026

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
17:35

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

Area of Science:

  • Immunology
  • Radiology
  • Pharmacology

Background:

  • Nonionic X-ray contrast media (CM) are widely used in computed tomography (CT) scans.
  • Hypersensitivity reactions to CM can occur, involving immunological pathways.
  • Basophils play a key role in allergic and hypersensitivity responses.

Purpose of the Study:

  • To investigate the impact of monomeric and dimeric nonionic CM on basophil degranulation.
  • To analyze the influence of immunological co-factors on CM-induced basophil activation.
  • To explore the relationship between CM administration and histamine release in patients.

Main Methods:

  • Analysis of plasma histamine levels and basophil CD63 expression via flow cytometry in 31 adult patients.
  • In vivo and in vitro studies were performed before and up to 24 hours after CM injection (iotrolan or iopromide).
  • Prestimulus analysis included Interleukin-3 (IL-3), Interleukin-1β (IL-1β), and N-formyl-methionyl-leucyl-phenylalanine (fMLF) treatments.

Main Results:

  • Iopromide showed a minimal increase in histamine, while iotrolan did not significantly alter levels.
  • A significant increase in basophil CD63 expression (activation marker) was observed post-CM injection (P<0.05).
  • IL-3 prestimulus enhanced CD63 expression more after iopromide than iotrolan; IL-1β effect appeared dose-dependent.

Conclusions:

  • Neither iopromide nor iotrolan inherently caused histamine release or basophil degranulation under normal conditions.
  • Individual patient stimuli significantly influence histamine release and CD63 expression, suggesting personalized hypersensitivity.
  • Flow cytometry analysis of CD63 expression is a valuable tool for understanding CM-mediated hypersensitivity reactions.