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Updated: Jun 13, 2026

Measuring the Behavioral Effects of Intraocular Scatter
Published on: February 18, 2021
Prevention of scattered light-induced asthenopia and fatigue by a polarized filter
Keiichi Hiramoto1, Yurika Yamate, Kumi Orita
1Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Abeno, Osaka, Japan. hiramoto@msic.med.osaka-cu.ac.jp
Background:
It has been well documented that a long-time irradiation of the eye by a strong light elicits eyestrain and fatigue. To elucidate the mechanism for the induction of light-induced fatigue and asthenopia, changes in the mouse were analyzed after white light-irradiation to the eye.
Methods:
C57BL/6j male mice were irradiated with white light in a specially designed room equipped with four mirrors covering all areas of its four walls to elicit diffused reflected light, and changes in their plasma levels of cortisol, INF-gamma, interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta) were analyzed.
Results:
Irradiation of mice with scattered white light significantly decreased the motional activity of animals, suggesting the occurrence of fatigue. Biochemical analysis and enzyme-immunoassay revealed that the irradiation of mice significantly elevated the plasma levels of cortisol, IFN-gamma, IL-10 and TGF-beta. All these changes were not observed with mice irradiated with the light in a similar room not equipped with mirrors. These changes were successfully inhibited by a polarized glass filter but not by a non-polarized filter with a similar absorbance.
Conclusions:
These observations suggest that irradiation of the eye by scattered reflected light stimulated a stress response via hypothalamo-pituitary-adrenal axis to enhance the secretion of cortisol from the adrenal grand and increase the plasma levels of cytokines.
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