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Analytical performance evaluation of a particle-enhanced turbidimetric cystatin C assay on the Roche COBAS 6000
Manuel Conde-Sánchez1, Esther Roldán-Fontana, Natalia Chueca-Porcuna
1U.G.C. Laboratorio Clínico, Hospital Nuestra Señora de la Merced, Avda. De la Constitución, 2. 41640-Osuna, Seville, Spain. manuel.conde.sanchez.sspa@juntadeandalucia.es
Objectives:
Cystatin C is a low molecular protein that has been proposed to estimate the glomerular filtration rate. Here we investigated the performance of the Roche cystatin C assay on the COBAS 6000 analyzer.
Design And Methods:
We studied the imprecision, recovery, limit of detection and quantification, linearity and interferences. For method comparison, split sample aliquots were assayed using the described method and a Siemens cystatin C assay.
Results:
The assay displayed a low total imprecision and a good linearity over the entire range tested. Bilirubin and triglycerides did not interfere with the assay, and only a haemoglobin concentration higher than 6g/dl interfered with the assay. The assay agreed well with the Siemens assay.
Conclusion:
The Roche cystatin C assay is an acceptable method for determining the cystatin C and the glomerular filtration rate estimate. On a COBAS 6000, the assay improves and simplifies the laboratory's workload.
