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Updated: Jun 13, 2026
High Precision Zinc Isotopic Measurements Applied to Mouse Organs
Published on: May 22, 2015
Quantitative determination of Zn protoporphyrin IX, heme and protoporphyrin IX in Parma ham by HPLC
Jun-Ichi Wakamatsu1, Hiroko Odagiri, Takanori Nishimura
1Meat Science Laboratory, Division of Bioresource and Product Science, Research Faculty of Agriculture, Hokkaido University, N-9 W-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan; Department of Animal Science, Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
Abstract:
We measured the contents of Zn protoporphyrin IX (ZPP), heme and protoporphyrin IX (PPIX) in Parma ham by simultaneous analysis using high-performance liquid chromatography (HPLC). Extraction with ethyl acetate-acetic acid (4:1) was suitable for the quantitative analysis of ZPP. The contents of heme, ZPP and PPIX in Parma ham were 15.0-29.9, 27.7-47.0 and 0.4-1.1μg/g, respectively, and total content of porphyrin was 43.7-76.6μg/g. The amount of ZPP in Parma ham was larger than that of heme, and ZPP accounted for 60-70% of all porphyrins.
