Related Experiment Video
Updated: Jun 13, 2026

Exploring the Longissimus Muscle: Unraveling its Correlation with Meat Quality in Bos indicus and Crossbred Bulls
Published on: July 12, 2024
Color stability and reversion in carbon monoxide packaged ground beef
Jong Youn Jeong1, James R Claus
1Meat Science and Muscle Biology Laboratory, University of Wisconsin-Madison, WI 53706, USA.
Carbon monoxide (CO) exposure enhances red meat color, but this effect diminishes after packaging. Longer CO exposure provides a slight, temporary color advantage for ground beef display.
Area of Science:
- Food Science
- Meat Science
- Color Chemistry
Background:
- Maintaining desirable red meat color during retail display is crucial for consumer acceptance.
- Carbon monoxide (CO) has been explored as a method to improve and stabilize meat color.
- Understanding the long-term color stability and reversion of CO-treated meat is essential for its commercial application.
Purpose of the Study:
- To investigate the impact of varying carbon monoxide (CO) exposure durations on the color stability of ground beef.
- To assess the color reversion of metmyoglobin to carboxymyoglobin in aged or low-oxygen packaged ground beef treated with CO.
- To determine the relationship between CO exposure length and carboxymyoglobin color loss during display.
Main Methods:
- Ground beef was exposed to carbon monoxide (CO) for 1, 2, 4, or 6 days.
- Color changes were monitored after repackaging in oxygen-permeable polyvinyl chloride (PVC) overwrap over 0, 24, 48, and 72 hours.
- The ability of CO to reverse the brown color of aged or low-oxygen modified ground beef was evaluated.
Main Results:
- Extended CO exposure duration led to increased initial redness in ground beef.
- Redness significantly decreased after packages were opened for display.
- Ground beef exposed to CO for 6 days retained only slightly higher redness than day 1 exposed beef after 72 hours.
- CO successfully converted brown metmyoglobin to red carboxymyoglobin in aged or low-oxygen induced ground beef.
Conclusions:
- While CO exposure enhances initial meat redness, the color benefit diminishes over time, especially after opening the package.
- CO can effectively reverse the brown color in aged or low-oxygen ground beef, forming carboxymyoglobin.
- The microbiological status of ground beef must be considered before CO packaging to ensure safety, despite its color reversion capabilities.
Related Concept Videos
Microbial Spoilage of Food
Methods of Controlling Food Spoilage
Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids
Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives
Drug Product Stability
Pasteurization and Food Preservation

