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Further characterization of volume regulatory decrease in cultured renal epitheloid (MDCK) cells.

M Ritter1, M Paulmichl, F Lang

  • 1Institute for Physiology, University of Innsbruck, Austria.

Pflugers Archiv : European Journal of Physiology
|March 1, 1991
PubMed
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Cell swelling in Madin Darby canine kidney cells causes membrane depolarization and reduced resistance. This indicates a transient potassium conductance increase followed by sustained anion conductance, impacting intracellular pH.

Area of Science:

  • Cell Physiology
  • Membrane Transport
  • Renal Cell Biology

Background:

  • Volume regulatory decrease (VRD) in Madin Darby canine kidney (MDCK) cells involves membrane hyperpolarization and depolarization.
  • Changes in cell membrane selectivity during VRD are not fully understood without membrane resistance (Rm) data.
  • Alterations in ion selectivity could stem from potassium conductance inhibition or anion conductance activation.

Purpose of the Study:

  • To determine the cell membrane resistance (Rm) during VRD in MDCK cells.
  • To elucidate the conductances responsible for observed changes in ion selectivity during VRD.
  • To investigate the role of chloride and bicarbonate in VRD-associated membrane potential changes.

Main Methods:

  • Cellular cable analysis was employed to measure Rm.

Related Experiment Videos

  • Three microelectrodes were used to inject current and record voltage deflections in a cluster of three MDCK cells.
  • Experiments were conducted using hypotonic perfusates, with and without chloride and bicarbonate.

    • Main Results:

    • Exposure to hypotonic perfusates caused a significant and sustained reduction in MDCK cell Rm.
    • The reduction in Rm was transient when chloride or bicarbonate and chloride were absent.
    • Cell swelling led to a transient increase in potassium conductance followed by a sustained increase in anion conductance.
    • Hypotonic perfusates decreased intracellular pH, potentially due to bicarbonate loss via anion channels.

    Conclusions:

    • Cell swelling in MDCK cells induces a decrease in membrane resistance, reflecting altered ion conductances.
    • VRD involves a transient increase in potassium conductance followed by a sustained increase in anion conductance.
    • Anion conductance may contribute to intracellular pH regulation during VRD through bicarbonate loss.