Detection of beta-glucans using an amperometric biosensor based on high-affinity interaction between Dectin-1 and...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Infectious Diseases

Background:

• Early diagnosis of fungal infections is crucial for effective antifungal therapy.
• Current diagnostic methods like cultures and histopathology are often slow and lack sensitivity.
• There is a need for rapid and sensitive diagnostic tools for fungal infections.

Purpose of the Study:

• To develop a sensitive amperometric biosensor for the rapid detection of beta-glucans.
• To utilize Dectin-1 immobilized on a novel multilayer film for beta-glucan detection.
• To evaluate the analytical performance and applicability of the biosensor for fungal infection screening.

Main Methods:

• Fabrication of a multilayer film using Nafion-thionine-gold nanoparticle-chitosan.
• Immobilization of Dectin-1 onto the multilayer film to create a biosensor.
• Detection of beta-glucans via monitoring electrochemical signal changes due to ligand-receptor complex formation.
• Optimization of biosensor conditions and assessment of accuracy, stability, and reproducibility.

Main Results:

• The fabricated biosensor demonstrated high sensitivity for beta-glucans.
• The biosensor exhibited good accuracy, stability, and reproducibility under optimal conditions.
• The detection mechanism relies on the inhibition of electron transmission upon beta-glucan binding.
• The biosensor successfully detected beta-glucans in serum samples.

Conclusions:

• The developed amperometric biosensor offers a rapid and sensitive method for beta-glucan detection.
• This biosensor can serve as a valuable tool for early screening and diagnosis of fungal infections.
• The proposed biosensor enhances the potential for timely antifungal therapeutic responses.