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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
Compilation of DNA sequences of Escherichia coli (update 1991)
1Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, FRG.
Abstract:
We have compiled the DNA sequence data for E. coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the third listing replacing and increasing the former listing roughly by one fifth. However, in order to save space this printed version contains DNA sequence information only. The complete compilation is now available in machine readable form from the EMBL data library (ECD release 6). After deletion of all detected overlaps a total of 1 492,282 individual bp is found to be determined till the beginning of 1991. This corresponds to a total of 31.62% of the entire E. coli chromosome consisting of about 4,720 kbp. This number may actually be higher by some extra 2.5% derived from lysogenic bacteriophage lambda and various DNA sequences already received for statistical purposes only.
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