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Distribution of different hepatitis C virus genotypes in patients with hepatitis C virus infection

Farah Bokharaei Salim1, Hossein Keyvani, Afsaneh Amiri

  • 1GI and Liver Disease Research Centre, Iran University of Medical Sciences, Firouzgar Hospital, Valadi Street, Valiasr Square, Tehran, Iran.

World Journal of Gastroenterology
|April 27, 2010
PubMed

Insights

Multiple hepatitis C virus (HCV) genotypes were detected in patients with chronic hepatitis C, with discrepancies observed between plasma, peripheral blood mononuclear cells (PBMCs), and liver biopsy specimens.

Area of Science:

  • Hepatology
  • Virology
  • Infectious Diseases

Background:

  • Chronic hepatitis C virus (HCV) infection is a significant global health concern.
  • Understanding HCV genotype distribution is crucial for effective antiviral therapy.
  • Mixed HCV infections can complicate diagnosis and treatment.

Purpose of the Study:

  • To investigate the prevalence of mixed HCV infections.
  • To determine discrepancies in HCV genotypes across different sample types: plasma, PBMCs, and liver biopsies.
  • To assess the impact of sample source on HCV genotype detection.

Main Methods:

  • Recruitment of 133 patients with chronic hepatitis C undergoing antiviral therapy.
  • Collection of plasma and peripheral blood mononuclear cells (PBMCs).
  • HCV genotyping performed on serum, PBMCs, and liver biopsy specimens using INNO-LiPA(TM) HCV II and confirmed by sequencing.

Main Results:

  • Multiple HCV genotypes were identified in 2.3% of plasma, 6.8% of PBMCs, and 18.2% of liver biopsy samples.
  • Significant discrepancies were noted in HCV genotypes detected between plasma, PBMCs, and liver biopsies.
  • The genotypes found in PBMCs differed from those in plasma and liver biopsy specimens.

Conclusions:

  • A notable proportion of chronic hepatitis C patients harbor multiple HCV genotypes.
  • HCV genotyping solely from serum may not capture the full complexity of mixed infections.
  • Liver biopsy and PBMC analysis can reveal HCV genotypes missed in plasma testing.
Abstract

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