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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Updated: Jun 13, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

A microarray-based genetic screen for yeast chronological aging factors.

Mirela Matecic¹, Daniel L Smith, Xuewen Pan

¹Department of Biochemistry and Molecular Genetics, University of Virginia Health System, School of Medicine, Charlottesville, Virginia, United States of America.

|April 28, 2010

Summary

This summary is machine-generated.

Budding yeast aging was studied using gene deletion mutants. Key findings reveal autophagy

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Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Published on: September 29, 2011

Area of Science:

Gerontology
Molecular Biology
Genetics

Background:

Model organisms are crucial for understanding aging.
Budding yeast (Saccharomyces cerevisiae) is a model for studying aging processes.
Chronological lifespan refers to the viability of non-dividing cells over time.

Purpose of the Study:

To identify genes regulating chronological lifespan in yeast.
To understand the role of specific cellular processes in aging.
To discover novel genetic factors influencing longevity.

Main Methods:

A large-scale screen of gene deletion mutants in Saccharomyces cerevisiae.
Chronological aging of pooled mutants in liquid culture.
DNA bar-code sequencing and microarray hybridization to quantify mutant abundance.

Main Results:

Identified multiple short- and long-lived mutants.
Autophagy (cellular recycling) is essential for yeast longevity.
Disrupting purine biosynthesis (ADE genes) extended lifespan, mimicking calorie restriction.
Acetic acid toxicity mechanism was modulated by identified mutants.

Conclusions:

Autophagy is critical for yeast chronological lifespan and response to nutrient limitation.
Inhibition of purine biosynthesis is a novel longevity-promoting pathway.
Identified genes provide new insights into aging mechanisms in yeast and potentially other organisms.