Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pleiotropic mutation in a tendril TCP gene underlies the yield-enhancing multiple-flowering trait in summer squash (Cucurbita pepo).

The Plant journal : for cell and molecular biology·2026

Same author

Targeting the "bitterness gene" by genome editing abolishes synthesis of bitter flavanones in citrus; prospects for new varieties and extended climates for cultivation.

The Plant journal : for cell and molecular biology·2025

Same author

The pomegranate (Punica granatum L. cv. 'Wonderful') genome and P. protopunica shed light on pomegranate domestication. Is Daru a wild stock?

BMC genomics·2025

Same author

Functional characterization of a ripening-specific L-methionine aminotransferase and its role in volatile C<sub>3</sub>-thioether esters biosynthesis in melon fruits.

Plant science : an international journal of experimental plant biology·2025

Same author

In planta genome editing in citrus facilitated by co-expression of CRISPR/Cas and developmental regulators.

The Plant journal : for cell and molecular biology·2025

Same author

Meta genetic analysis of melon sweetness.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2025

Same journal

Genome-wide analysis across Indian camel populations reveals genetic distinctiveness of the Kharai camel breed.

BMC genomics·2026

Same journal

Different genomic footprint of small insertion-deletion and structural variants determines the genetic divergence of indica and japonica rice.

BMC genomics·2026

Same journal

From nurse bee to queen egg: RNA-seq analysis of Apis mellifera eggs shows dietary protein-dependent gene regulation.

BMC genomics·2026

Same journal

A genome-wide association study to identify the genetic loci underlying carbapenem resistance in Acinetobacter baumannii.

BMC genomics·2026

Same journal

Comparative transcriptome analysis to reveal key drought stress-responsive genes in sorghum (Sorghum bicolor (L.) Moench).

BMC genomics·2026

Same journal

Tissue identity is the dominant determinant of cross-species transferability of a porcine developmental programme.

BMC genomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

High-throughput marker discovery in melon using a self-designed oligo microarray.

Ron Ophir¹, Ravit Eshed, Rotem Harel-Beja

¹Plant Sciences Institute, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel.

|April 30, 2010

Summary

This summary is machine-generated.

Researchers discovered thousands of new single-feature polymorphisms (SFPs) in melon using a custom microarray. These SFPs are valuable for genetic mapping and accelerating crop improvement in breeding programs.

More Related Videos

Polymer Microarrays for High Throughput Discovery of Biomaterials

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

Related Experiment Videos

Last Updated: Jun 13, 2026

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Polymer Microarrays for High Throughput Discovery of Biomaterials

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

Area of Science:

Plant genomics
Agricultural biotechnology
Molecular genetics

Background:

Genetic maps are crucial for crop breeding programs, but marker discovery is often a bottleneck, especially in crops with limited genomic resources like melon.
Microarray-based DNA hybridization can identify single-feature polymorphisms (SFPs), which serve as valuable molecular markers for genetic analysis and breeding.

Purpose of the Study:

To develop and apply a modified microarray approach for high-throughput marker discovery in melon, a crop with limited genomic resources.
To identify novel single-feature polymorphisms (SFPs) for use in melon genetic mapping and breeding programs.

Main Methods:

Designed and utilized a custom oligonucleotide microarray based on a partial expressed sequence tag (EST) collection of melon.
Hybridized DNA from parental lines of a mapping population to the microarray to identify putative single-feature polymorphisms (SFPs).
Validated identified SFPs through sequencing and tested their conservation in another melon mapping population.

Main Results:

Discovered 6,184 putative single-feature polymorphisms (SFPs) between the parents of the mapping population.
Sequencing validation of 245 SFPs demonstrated a sensitivity of approximately 79%, with 81% of SFPs being single-nucleotide polymorphisms (SNPs).
Testing on an additional mapping population confirmed the conservation of many identified SFPs across different melon genetic backgrounds.

Conclusions:

Developed a custom oligo microarray approach that successfully discovered thousands of novel SFPs for melon genetic mapping and molecular-assisted breeding.
A significant portion of the discovered SFPs are conserved, making them applicable to diverse melon breeding populations.
This microarray-based SFP discovery method is a viable strategy for crops with limited genomic resources, despite the need for further improvements in discovery rates.