Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the others.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probing Li-ion conduction in glassy Li<sub>2</sub>S-P<sub>2</sub>S<sub>5</sub> electrolytes using NMR.

Solid state nuclear magnetic resonance·2026
Same author

Moving NMR infrastructures to remote access capabilities.

Progress in nuclear magnetic resonance spectroscopy·2026
Same author

Equivariant machine learning of electric field gradients-Predicting the quadrupolar coupling constant in the MAPbI3 phase transition.

The Journal of chemical physics·2025
Same author

Transforming solid-state nuclear magnetic resonance towards a chemistry-ready technique.

Solid state nuclear magnetic resonance·2025
Same author

Automated wide-line nuclear quadrupole resonance of mixed-cation lead-halide perovskites.

Magnetic resonance (Gottingen, Germany)·2025
Same author

Quantitative Trace Analysis of Dilute Mixtures Using a Benchtop NMR System with SABRE Hyperpolarization.

Analytical chemistry·2025

Related Experiment Video

Updated: Jun 13, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Full quadrupolar tensor determination by NMR using a micro-crystal spinning at the magic angle.

Suresh Kumar Vasa1, Ernst R H van Eck, J W G Janssen

  • 1Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.

Physical Chemistry Chemical Physics : PCCP
|April 30, 2010
PubMed
Summary

This study introduces rotor-synchronized Magic Angle Spinning (MAS) NMR for determining quadrupolar coupling tensor values in half-integer nuclei. Researchers successfully measured the complete quadrupolar tensor for sodium-23 in a microcrystal of sodium nitrate.

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Magnetic Tweezers for the Measurement of Twist and Torque
11:41

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

Related Experiment Videos

Last Updated: Jun 13, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Magnetic Tweezers for the Measurement of Twist and Torque
11:41

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Materials Science
  • Quantum Chemistry

Background:

  • Quadrupolar coupling tensor values are crucial for understanding the electronic environment of half-integer nuclei.
  • Traditional methods for determining these tensors often require large single crystals, limiting their applicability.
  • Microcrystalline samples present a challenge for high-resolution NMR studies due to their small size and lower signal-to-noise ratio.

Purpose of the Study:

  • To implement and validate rotor-synchronized Magic Angle Spinning (MAS) NMR for precise determination of quadrupolar coupling tensor values.
  • To demonstrate the capability of this technique for analyzing microcrystalline samples.
  • To determine the full quadrupolar tensor of sodium-23 (23Na) in sodium nitrate (NaNO3) using a microcrystal.

Main Methods:

  • Development and implementation of rotor-synchronized Magic Angle Spinning (MAS) NMR.
  • Utilization of a microcoil-based probehead for enhanced sensitivity with microcrystalline samples.
  • A two-step spectral simulation procedure to extract quadrupolar tensor orientation.
  • X-ray Diffraction (XRD) analysis for experimental verification.

Main Results:

  • Successful determination of the complete quadrupolar coupling tensor for 23Na in a microcrystal of NaNO3.
  • Demonstration of superior sensitivity and resolution using a microcoil probehead for microcrystals.
  • Validation of the obtained tensor orientation through comparative XRD analysis.

Conclusions:

  • Rotor-synchronized MAS NMR is a powerful technique for characterizing quadrupolar interactions in microcrystalline samples.
  • The developed method enables accurate determination of quadrupolar coupling tensor values for half-integer nuclei.
  • This approach significantly expands the scope of solid-state NMR for materials characterization.