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Updated: Jun 13, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
08:16

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

Published on: February 1, 2014

Production and characterisation of monoclonal antibodies specific for Escherichia coli O157:H7

M Luciani1, G Armillotta, M Magliulo

  • 1Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Campo Boario, 64100 Teramo, Italy.

Veterinaria Italiana
|April 30, 2010
PubMed

Seven monoclonal antibodies (MAbs) specific for Escherichia coli O157:H7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising Balb/c mice with the strain E. coli O157:H7. These monoclonal antibodies do not cross-react with other bacteria such as Salmonella enterica serovar Typhimurium, E. coli O14, E. coli JM109, S. enterica serovar Enteritidis, S. panama, S. saintpaul, S. derby, S. muenchen, S. bredeney, S. hadar, Yersinia enterocolitica, Proteus vulgaris, Shigella flexneri, Listeria ivanovii, L. monocytogenes 13M, L. innocua, Enterobacter cloacae, E. agglomerans, E. amnigenus, Citrobacter freundii, Escherichia fergussoni or Klebsiella pneumoniae. Of the seven MAbs obtained, MAb 8B8C3 was selected to prepare a high-sensitivity sandwich ELISA method specific for O157:H7.

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