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[A comparative study on HPLC fingerprint of Semen Strychni and the processed]
Jun-song Li1, Dan-dan Wang, Bao-chang Cai
1Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of TCM, Nanjing 210029, China. lijunsong1964@163.com
Objective:
To establish the fingerprint of Semen Strychni and the processed by HPLC.
Methods:
The HPLC Method was used, the chromatgraphic conditions were as follows: Chromatographic column: Lichrospher C18 (250 mm x 4.6 mm, 5 microm), mobile phase: solvent A consisted of water-ethanoic acid-triethylamine (100: 0.2: 0.2), solvent B contained acetonitril (gradient elution), column temperature: 30 degrees C, flow rate: 1.0 mL/min, detection wavelength: 254 nm.
Results:
The similar degree of 10 batch samples buiding sharing mode of crude drug and processed product was above 0.9. 18 common peaks in chromatograms were separated from 10 batches of Semen Strychni samples and 21 common peaks in chromatograms were separated from 10 batches of the processed.
Conclusion:
This study establishes the HPLC fingerprint commonmode, and researches the difference between Semen Strychni and the processed in HPLC fingerprint.
