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[Preparation and authentication of beta-cyclodextrin complex of volatile oil from Ledum palustre]
1College of Pharmacy, Yanbian University, Yanji 133000, China.
Objective:
To study the optimum inclusion process conditions for the beta-cyclodextrin complex of volatile oil from Ledum palustre.
Methods:
The utilization ratio of the volatile oil from Ledum palustre was set as the index, and four factors were selected including the ratio of the volatile oil to beta-cyclodextrin, the temperature of preparation, the inclusion time and the ratio of beta-cyclodextrin to water. The optimum conditions were investigated by the orthogonal test form L9 (3(4)). The inclusion compound was identified by the ways of Micro-imaging, Thin-layer chromatography (TLC), Ultraviolet Spectroscopy (UV)and Infrared Spectroscopy (IR).
Results:
The optimum preparation conditions were as follows: volatile oils: beta-cyclodextrin was 1:8, the inclusion temperature and time was 50 degrees C and 30 min, respectively. Beta-cyclodextrin: water was 1:15.
Conclusion:
The inclusion technology is stable and its quality is adjustable.
