Related Experiment Video
Updated: Jun 13, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[Studies on technology optimization for extracting triterpenoid saponins from Picria felterrae by multi-target
Hong Fang1, De-sheng Ning, Xiao-yan Liang
1Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Academia Sinica, Guilin 541006, China. GXZWSFH@yahoo.cn
Objective:
To study the optimum extraction conditions of triterpenoid saponins from Picria felterrae.
Methods:
The optimum extraction conditions were investigated by the contents of the total glycosides and picfeltarraenine I A and I B, using orthogonal test.
Results:
The optimum extraction conditions were as follows: using 70% alcohol of 19 times than the amount of original material soaking 3 hours, extracting twice with hot reflux method at 80 degrees C, 3h the first time, 1 h the second time.
Conclusion:
The selected extraction conditions are convenient and practical with high accuracy, and could be used as a reference for industrial production.