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High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
Published on: May 10, 2016
Cellulose of Salicornia brachiata
Naresh D Sanandiya1, Kamalesh Prasad, Ramavatar Meena
1Central Salt & Marine Chemicals Research Institute, Council of Scientific & Industrial Research (CSIR), Bhavnaga, Gujarat, India.
Abstract:
Cellulose was extracted from the roots, stems and stem tips of Salicornia brachiata Roxb. Each crude cellulose sample obtained was fractionated into alpha- and beta-celluloses. The yields of crude cellulose from the stems and stem tips were greatest and lowest, respectively, while the yields of alpha- and beta-celluloses were in the order, roots > stems > stem tips. The cellulose samples were characterized by Fourier transform infrared spectroscopy (FT-IR), solid state cross polarisation magic angle spinning carbon-13 nuclear magnetic resonance spectroscopy (CP/MAS 13C NMR), X-ray diffraction pattern (XRD), thermo gravimetric analysis (TGA) and scanning electron microscopy (SEM). The data were compared with those of the celluloses (predominantly alpha-cellulose) isolated from Whatman filter paper No. 4 (WFP).
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