Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid DNA sequencing based upon single molecule detection.

L M Davis1, F R Fairfield, C A Harger

  • 1Center for Human Genome Studies, Los Alamos National Laboratory, NM 87545.

Genetic Analysis, Techniques and Applications
|February 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and acquired immunologic responses to West Nile virus infection of domestic chickens (Gallus gallus domesticus).

Poultry science·2011
Same author

Ray tracing analysis of the image quality of a high collection efficiency mirror system.

Applied optics·2010
Same author

Automated single-cell manipulation and sorting by light trapping.

Applied optics·2010
Same author

Nature's Effort to Cure an Appendicitis.

The Ulster medical journal·2010
Same author

The power profile predicts road cycling MMP.

International journal of sports medicine·2010
Same author

Evaluation of Bacillus anthracis and Yersinia pestis sample collection from nonporous surfaces by quantitative real-time PCR.

Letters in applied microbiology·2010
Same journal

High-throughput rapid yeast DNA extraction. Application to yeast artificial chromosomes as polymerase chain reaction templates.

Genetic analysis, techniques and applications·1994
Same journal

Improved single-strand conformation polymorphism analysis by asymmetric polymerase chain reaction with end-labeled primers.

Genetic analysis, techniques and applications·1994
Same journal

Kinetic determination of cellular LacZ expression.

Genetic analysis, techniques and applications·1994
Same journal

Competitive polymerase chain reaction and analysis of viral activity at the molecular level.

Genetic analysis, techniques and applications·1994
Same journal

Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture.

Genetic analysis, techniques and applications·1994
Same journal

Screening cosmid libraries with oligonucleotides corresponding to splice-site consensus sequences.

Genetic analysis, techniques and applications·1994
See all related articles

A new laser-based DNA sequencing method rapidly analyzes large DNA fragments (40-kb+). This technique accelerates genomic research by improving the speed and efficiency of DNA sequencing.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Current DNA sequencing methods can be time-consuming and labor-intensive, especially for large DNA fragments.
  • Assembling large genomic sequences often requires extensive subcloning and numerous overlapping sequences.

Purpose of the Study:

  • To develop a novel laser-based technique for rapid, high-throughput sequencing of large DNA fragments (40-kb or larger).
  • To enhance the efficiency of genomic sequence assembly by reducing the need for subcloning and overlapping sequences.

Main Methods:

  • Fluorescent labeling of bases within a single, large DNA fragment.
  • Immobilization of the labeled DNA fragment onto a support.
  • Introduction of the supported DNA into a flowing sample stream.

Related Experiment Videos

  • Detection of fluorescently labeled bases as they are enzymatically cleaved by an exonuclease.

    • Main Results:

    • The developed technique achieves DNA sequencing rates of 100 to 1000 bases per second.
    • Demonstrated capability to sequence DNA fragments of 40-kilobases or larger.

    Conclusions:

    • This laser-based approach offers a significant advancement in DNA sequencing technology.
    • The ability to rapidly sequence large DNA fragments will streamline the process of assembling large-scale genomic information.