Purification and properties of a nucleoside diphosphosugar: NAD+ 2-hexosyl oxidoreductase

Penicillium charlesii contains a nucleoside diphosphosugar: NAD 2-hexosyl oxidoreductase that oxidizes UDPgalactose (UDP-Galp), ADPribose [1,2] and UDPglucose (UDP-Glc). Dithiothreitol, NAD and 0.25 M NaCl but not nucleoside diphosphates stabilize the enzyme activity. The enzyme was purified and separated on polyacrylamide disc gels by electrophoresis into one major and eight minor bands of protein. Oxidoreductase activity was located in the major and three of the minor bands of protein. Each of these proteins catalyze the oxidation of UDPGalp, ADPribose and UDP-Glc.