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Related Experiment Video

Updated: Jun 13, 2026

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

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Published on: October 21, 2022

What influences DNA replication rate in budding yeast?

Thomas W Spiesser¹, Christian Diener, Matteo Barberis

¹Theoretical Biophysics, Institute for Biology, Humboldt University Berlin, Berlin, Germany.

|May 4, 2010

Summary

DNA replication time in yeast is influenced by both global rates and specific genomic regions. Our study quantifies sequence properties affecting DNA synthesis, revealing localized variations in replication speed.

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Area of Science:

Molecular Biology
Genetics
Computational Biology

Background:

DNA replication initiates at origins, with helicase and polymerase processing DNA filaments.
Replication forks are sites of DNA synthesis; fork speed influences initiation density.
Causes of varying DNA replication times remain unclear, despite potential epigenetic, transcriptional, and nucleotide factors.

Purpose of the Study:

Quantify the contribution of sequence properties to DNA replication time in budding yeast.
Differentiate between sequence-specific and sequence-independent influences on replication timing.
Develop predictive models for DNA replication dynamics.

Main Methods:

Interpreted DNA replication as a directed random walk.
Decomposed replication time influences into sequence-specific and independent components.
Analyzed genomic landscapes for replication rate variations.

Main Results:

Replication elongation time is largely explained by a global average rate for much of the genome.
Identified genomic regions with highly specific replication rates not explained by global factors.
Characterized genomic regions with altered elongation characteristics independent of initiation or sequence composition.

Conclusions:

Computational models for predicting DNA replication dynamics in yeast are scarce.
Replication time is governed by factors beyond polymerase movement along DNA.
This work enables characterization of genomic regions with distinct elongation properties.