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Updated: Jun 13, 2026

In vivo Neuronal Calcium Imaging in C. elegans
11:06

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Published on: April 10, 2013

Functional multineuron calcium imaging for systems pharmacology.

Naoya Takahashi1, Yuji Takahara, Daisuke Ishikawa

  • 1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Analytical and Bioanalytical Chemistry
|May 4, 2010
PubMed
Summary

Functional multineuron calcium imaging (fMCI) reveals brain activity at the single-neuron level. This technique aids research into neurological diseases and drug actions, offering insights into disorder mechanisms and therapeutic targets.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Computational Biology

Background:

  • Functional multineuron calcium imaging (fMCI) enables large-scale, single-neuron resolution of brain activity.
  • It visualizes neuronal function by detecting action potential-evoked calcium transients within neuron cell bodies.

Purpose of the Study:

  • To highlight fMCI as a high-throughput tool for studying brain diseases.
  • To demonstrate its utility in assessing pharmacological effects on neuronal networks.
  • To underscore its potential in uncovering neurological disorder mechanisms and identifying therapeutic targets.

Main Methods:

  • Utilizes calcium imaging to monitor neuronal activity.
  • Focuses on detecting transient calcium influxes associated with action potentials.

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Last Updated: Jun 13, 2026

In vivo Neuronal Calcium Imaging in C. elegans
11:06

In vivo Neuronal Calcium Imaging in C. elegans

Published on: April 10, 2013

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm
08:42

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Published on: October 4, 2018

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  • Applies fMCI in animal models of neurological conditions.

    • Main Results:

    • fMCI has been successfully applied to study altered neuronal activity in models of stroke, Alzheimer's disease, and epilepsy.
    • The technique allows estimation of how pharmacological agents influence both healthy and diseased neuronal networks.

    Conclusions:

    • fMCI provides a powerful method for investigating brain function at the single-neuron level.
    • It offers significant opportunities for advancing our understanding of neurological disorders.
    • fMCI can accelerate the discovery of novel therapeutic strategies for brain diseases.