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Updated: Jun 13, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Genetic introduction of foreign genes to Pleurotus eryngii by restriction enzyme-mediated integration
Won Noh1, Sang-Woo Kim, Bae Dong-Won
1Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Chinju, 660-701, Republic of Korea.
Abstract:
Pleurotus eryngii was transformed via restriction enzyme-mediated integration. In order to construct the transformation plasmid, the enhanced cyan fluorescent protein (ECFP) gene was ligated next to the gpd promoter of the plasmid pAN7-1. Transformation was facilitated via the heat treatment of a transformation mixture containing 1 microg of the HindIII-digested plasmid DNA and 10(6) mushroom protoplasts in 40% polyethyleneglycol solution, resulting in 10-40 hygromycin-resistant transformants. Successful transformation was evidenced by PCR, Southern blot, and confocal fluorescence microscopic analyses on the selected transformants. To date, this is the first report on the transformation of P. eryngii by REMI technique.
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