Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil Transcriptomic Changes in Severe Sterile Vasoplegic Syndrome Resemble a Distinct Molecular Subtype of Septic Shock.

Shock (Augusta, Ga.)·2026
Same author

Relationship among Toll-like receptors, β2 adrenergic receptor, and mitogen-activated protein kinases in regulating Il1b transcription.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Stepwise neofunctionalization of the NF-κB family member Rel during vertebrate evolution.

Nature immunology·2025
Same author

NF-κB RelB suppresses the inflammatory gene expression programs of dendritic cells by competing with RelA for binding to target gene promoters.

Cell discovery·2025
Same author

Examining NF-κB genomic interactions by ChIP-seq and CUT&Tag.

bioRxiv : the preprint server for biology·2024
Same author

Selective regulation of a defined subset of inflammatory and immunoregulatory genes by an NF-κB p50-IκBζ pathway.

Genes & development·2024
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Oct 4, 2025

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts &#8212; Implications for Neurodegenerative Disease Pathogenesis
09:09

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

2.6K

Luciferase assay.

Stephen T Smale

    Cold Spring Harbor Protocols
    |May 5, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Quantify promoter strength using luciferase reporter assays. This method measures enzyme activity in transfected cells, providing a sensitive and rapid assessment of gene expression levels.

    More Related Videos

    A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
    10:13

    A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

    Published on: July 3, 2013

    11.3K
    Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
    13:48

    Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

    Published on: September 7, 2008

    13.0K

    Related Experiment Videos

    Last Updated: Oct 4, 2025

    Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts &#8212; Implications for Neurodegenerative Disease Pathogenesis
    09:09

    Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

    Published on: October 11, 2024

    2.6K
    A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
    10:13

    A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

    Published on: July 3, 2013

    11.3K
    Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
    13:48

    Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

    Published on: September 7, 2008

    13.0K

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Quantifying promoter strength is crucial for transfection assays.
    • Low transfection efficiency necessitates reporter genes for accurate measurement.
    • Reporter protein activity correlates with steady-state mRNA levels.

    Purpose of the Study:

    • To outline a protocol for quantifying promoter strength using the luciferase reporter gene.
    • To detail the methodology for measuring luciferase activity in transfected cells.

    Main Methods:

    • Transfection of cells with a luciferase reporter plasmid.
    • Cell lysis using a detergent-containing buffer.
    • Measurement of luciferase activity via luminometry after substrate addition.

    Main Results:

    • Luciferase activity provides a sensitive measure of promoter strength.
    • The assay is rapid, simple, inexpensive, and has a broad linear range.
    • Automated and manual luminometry methods offer consistent results.

    Conclusions:

    • The luciferase reporter assay is a highly effective tool for quantifying promoter strength in gene expression studies.
    • This protocol offers a reliable and sensitive method for assessing gene regulation.