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Related Experiment Video

Updated: Jun 13, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

Stabilization of proteins for storage.

Richard J Simpson

Cold Spring Harbor Protocols

|May 5, 2010

Summary

Maintaining protein stability during long-term storage is crucial for proteomics research and pharmaceutical applications. Proper storage conditions, including temperature, pH, and additives like glycerol, are essential to prevent protein inactivation and aggregation.

Area of Science:

Biochemistry
Proteomics
Pharmaceutical Science

Background:

Proteins and peptides require long-term storage after purification for subsequent analyses.
Maintaining biological function and preventing inactivation are critical for protein pharmaceuticals and research samples.
Protein shelf-life is influenced by intrinsic properties and extrinsic storage conditions.

Purpose of the Study:

To discuss major causes of protein inactivation.
To describe measures for maintaining protein stability and solubility during storage.

Main Methods:

Review of factors affecting protein stability.
Discussion of storage conditions (temperature, pH).
Exploration of stabilizing additives (e.g., glycerol, sucrose).

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Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Published on: January 28, 2015

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Last Updated: Jun 13, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

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Published on: February 11, 2019

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Published on: January 28, 2015

Main Results:

Protein inactivation is caused by various intrinsic and extrinsic factors.
Appropriate storage conditions are vital for preserving protein activity.
Additives like glycerol and sucrose can prevent aggregation and maintain protein function.

Conclusions:

Optimal storage strategies are necessary to ensure protein integrity over time.
Understanding protein inactivation mechanisms aids in developing effective stabilization methods.
Proper handling and storage are key for reliable downstream applications in proteomics and pharmaceuticals.