Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

COP Coated Vesicles00:59

COP Coated Vesicles

Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homopolymers as structure-driving agents in semicrystalline block copolymer micelles.

ACS nano·2015
Same author

Control of morphology and corona composition in aggregates of mixtures of PS-b-PAA and PS-b-P4VP diblock copolymers: effects of solvent, water content, and mixture composition.

Langmuir : the ACS journal of surfaces and colloids·2014
Same author

"Raft" formation by two-dimensional self-assembly of block copolymer rod micelles in aqueous solution.

Angewandte Chemie (International ed. in English)·2014
Same author

Control of corona composition and morphology in aggregates of mixtures of PS-b-PAA and PS-b-P4VP diblock copolymers: effects of pH and block length.

Langmuir : the ACS journal of surfaces and colloids·2014
Same author

Bacteria survival probability in bactericidal filter paper.

Colloids and surfaces. B, Biointerfaces·2014
Same author

Crystallinity-driven morphological ripening processes for poly(ethylene oxide)-block-polycaprolactone micelles in water.

Soft matter·2014
Same journal

Interfacial Behavior and Adsorption Mechanisms of Sorbitol Polyether Ester Emulsifiers in D-Phase Emulsion Systems: Applications for Spontaneous Emulsification.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Ti<sub>3</sub> Cluster-Doped 2D Goldene Surface for Electronic and Optical Sensing of Oxygen, Nitrogen, and Hydrocarbon-Based Respiratory Biomarkers: A DFT Study.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Condensation of GO<i>x</i> by Restoring Global Protein Fold on Diazo-Enriched SG Surfaces: Reinstallation of FADs Restores Biocatalytic Sustainability in Quantifying Blood Glucose.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Correction to "Protein Encapsulated DNA Metal Nanocluster as Fluorescent Nanoprobe for Detecting Insulin".

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Rational Design of V<sub>2</sub>O<sub>5</sub> Hierarchical Microspheres with Tunable Porosities and Primary Building Blocks for Enhanced Lithium Storage Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Roles of Anion and Cation Doping in g-C<b><sub>3</sub></b>N<b><sub>4</sub></b> as Artificial SEI for Regulating Interfacial Zn<b><sup>2+</sup></b> Deposition in Aqueous Zinc Metal Anodes.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
11:42

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

Published on: January 22, 2015

Fully collapsed (kippah) vesicles: preparation and characterization.

Tony Azzam1, Adi Eisenberg

  • 1Department of Chemistry and Centre for Self Assembled Chemical Structures, McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 2K6, Canada.

Langmuir : the ACS Journal of Surfaces and Colloids
|May 7, 2010
PubMed
Summary
This summary is machine-generated.

The formation of novel kippah vesicles from poly(acrylic acid)-block-polystyrene (PAA-b-PS) copolymers is highly dependent on preparation methods, specifically freeze-drying with rehydration or vacuum drying, leading to thicker-walled hemispherical structures.

More Related Videos

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Published on: July 13, 2013

Using the Droplet Transfer Method to Reliably Prepare Giant Unilamellar Vesicles
08:53

Using the Droplet Transfer Method to Reliably Prepare Giant Unilamellar Vesicles

Published on: September 19, 2025

Related Experiment Videos

Last Updated: Jun 13, 2026

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
11:42

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

Published on: January 22, 2015

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Published on: July 13, 2013

Using the Droplet Transfer Method to Reliably Prepare Giant Unilamellar Vesicles
08:53

Using the Droplet Transfer Method to Reliably Prepare Giant Unilamellar Vesicles

Published on: September 19, 2025

Area of Science:

  • Polymer science
  • Materials science
  • Nanotechnology

Background:

  • Amphiphilic block copolymers self-assemble into various nanostructures, including vesicles.
  • Understanding vesicle morphology is crucial for applications in drug delivery and nanotechnology.
  • New morphologies, like the 'kippah', expand the known structural diversity of polymer vesicles.

Purpose of the Study:

  • To investigate the formation of a novel hemispherical vesicle structure, termed 'kippah'.
  • To determine the influence of different specimen preparation methods on vesicle morphology.
  • To characterize the structural properties of kippah vesicles using transmission electron microscopy (TEM).

Main Methods:

  • Preparation of poly(acrylic acid)-block-polystyrene (PAA-b-PS) copolymer vesicles (approx. 500 nm diameter).
  • Vesicle specimen preparation using four methods: ambient drying, freeze-drying, freeze-drying/rehydration, and vacuum drying.
  • Analysis of vesicle morphology using transmission electron microscopy (TEM) and specimen tilting.

Main Results:

  • Kippah vesicle formation was exclusively observed with freeze-drying followed by rehydration or direct vacuum drying.
  • Ambient drying and direct freeze-drying yielded 'normal' vesicles with classical indentation patterns.
  • Kippah vesicles exhibited a nearly round appearance with approximately twice the wall thickness of normal vesicles.
  • Specimen tilting aided in analyzing kippah structure and determining orientation (open-side-up/down).

Conclusions:

  • The preparation method critically controls the formation of kippah vesicles from PAA-b-PS copolymers.
  • Factors such as wall flexibility, pressure gradients, and surface tension are postulated to influence kippah formation.
  • The study highlights the importance of controlled drying and rehydration techniques in vesicle morphology.
  • Kippah vesicles represent a distinct morphology with potential implications for materials science and nanotechnology.