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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

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Updated: Jun 13, 2026

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

Core-shell diamond as a support for solid-phase extraction and high-performance liquid chromatography.

Gaurav Saini1, David S Jensen, Landon A Wiest

  • 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602, USA.

Analytical Chemistry
|May 8, 2010
PubMed
Summary
This summary is machine-generated.

Core-shell diamond particles were synthesized using layer-by-layer deposition for enhanced solid-phase extraction and high-performance liquid chromatography applications. These novel diamond materials exhibit improved surface area and analyte loading capacities.

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Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
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High Pressure Single Crystal Diffraction at PX^2
11:32

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) are crucial analytical techniques.
  • Developing novel stationary phases with enhanced properties is essential for improving separation efficiency and analyte detection.
  • Diamond-based materials offer unique chemical and physical properties suitable for chromatographic applications.

Purpose of the Study:

  • To synthesize core-shell diamond particles using layer-by-layer (LbL) deposition for SPE and HPLC.
  • To characterize the morphology, surface area, and pore size of the synthesized core-shell diamond particles.
  • To evaluate the performance of these particles as stationary phases in SPE and HPLC.

Main Methods:

  • Core-shell diamond particles were fabricated via LbL deposition of polyallylamine (PAAm) and nanodiamond onto microdiamond cores.
  • Cross-linking was achieved using 1,2,5,6-diepoxycyclooctane or 1,2-epoxyoctadecane.
  • Characterization involved scanning electron microscopy (SEM), Rutherford backscattering spectrometry (RBS), nuclear reaction analysis (NRA), diffuse reflectance infrared Fourier transform spectroscopy (DRIFT), environmental scanning electron microscopy (ESEM), and Brunauer-Emmett-Teller (BET) analysis.
  • SPE and HPLC performance were assessed using model analytes.

Main Results:

  • Larger core-shell diamond particles (approx. 50 microm) demonstrated significantly higher surface areas and analyte loading capacities for SPE compared to nonporous diamond particles.
  • Smaller core-shell diamond particles (approx. 3 microm) showed excellent performance in both normal and reversed-phase HPLC.
  • High plate numbers were achieved, such as 36,300 plates/m for mesitylene and 54,800 plates/m for diazinon.

Conclusions:

  • LbL deposition is an effective method for creating core-shell diamond particles with tunable properties.
  • These core-shell diamond particles represent promising new materials for advanced SPE and HPLC applications.
  • The enhanced surface area and chromatographic performance highlight the potential of engineered diamond nanomaterials in analytical separations.