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Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31
T Matsunaga1, H Mihashita, M Miyake
1Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
FEBS Letters
|June 3, 1991
Abstract:
The minimum region required for replication of the marine Rhodobacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.