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Preparation of Functional Silica Using a Bioinspired Method
Published on: August 1, 2018
Novel microbiosensors prepared utilizing biomimetic silicification method
Faming Tian1, Wenjue Wu, Michael Broderick
1Biosensor Group, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Biosensors & Bioelectronics
|May 8, 2010
Summary
We developed a simple biomimetic silicification method using poly-L-lysine to create enzyme-based microbiosensors for detecting various analytes. This efficient technique allows for robust enzyme entrapment and demonstrates good performance for adenosine detection.
Area of Science:
- Biomaterials Science
- Biosensor Technology
- Electrochemistry
Background:
- Enzyme entrapment is crucial for microbiosensor fabrication.
- Existing methods can be harsh or inefficient.
- A mild, efficient method is needed for robust enzyme immobilization.
Purpose of the Study:
- To develop a simple, rapid, and mild biomimetic silicification method for microbiosensor fabrication.
- To immobilize enzymes for detecting various analytes.
- To fabricate and characterize adenosine microbiosensors.
Main Methods:
- Functionalization of Pt microelectrodes with poly(pyrrole-1-propanoic acid).
- Covalent immobilization of poly-L-lysine using EDC/NHSS cross-linkers.
- Biomimetic silicification catalyzed by poly-L-lysine for enzyme entrapment.
- Fabrication of adenosine, l-glutamate, and lactate microbiosensors.
Main Results:
- Simultaneous entrapment of adenosine deaminase (AD), nucleoside phosphorylase (NP), and xanthine oxidase (XO) enzymes.
- Adenosine biosensors showed high sensitivity (153.0+/-2.4 microA mM(-1)cm(-2)), low detection limit (40 nM), and fast response time (25+/-2s).
- Effective selectivity against interfering substances using a poly(diaminobenzene) screening barrier.
Conclusions:
- The biomimetic silicification method is a mild and efficient approach for microbiosensor fabrication.
- The method is adaptable for various enzymes and analytes, including l-glutamate and lactate.
- This technique offers a promising platform for developing advanced biosensing devices.
