DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases
Nan Dai1, Yin Nah Teo, Eric T Kool
1Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
Chemical Communications (Cambridge, England)
|May 8, 2010
Abstract:
DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.
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