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Convergent solid-phase peptide synthesis. VIII. Synthesis, using a photolabile resin, and purification of a
P Lloyd-Williams1, F Albericio, E Giralt
1Department of Organic Chemistry, University of Barcelona, Spain.
International Journal of Peptide and Protein Research
|January 1, 1991
Abstract:
The solid-phase synthesis, photolytic detachment from the solid support and purification in solution, of a fully-protected octapeptide containing a methionine residue (protected as the sulphoxide) is described. Protection of methionine in this manner avoids problems associated with the oxidation of this residue during the photolysis. The peptide has been purified by medium pressure liquid chromatography using solvent mixtures containing a high proportion of dimethylformamide in order to avoid precipitation of the peptide on the column.