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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview

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Related Experiment Video

Updated: Jun 13, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Organization of DNA replication.

Vadim O Chagin1, Jeffrey H Stear, M Cristina Cardoso

  • 1Department of Biology, Technische Universität Darmstadt, Germany.

Cold Spring Harbor Perspectives in Biology
|May 11, 2010
PubMed
Summary
This summary is machine-generated.

Understanding DNA replication has advanced from its double helix discovery to in vivo cellular organization. New high-throughput and super-resolution microscopy techniques now allow real-time visualization of genome duplication within cells.

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Last Updated: Jun 13, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The DNA double helix structure discovery implied semi-conservative replication.
  • Early research identified key enzymes and mechanisms through genetic and biochemical methods.
  • Recent focus shifts to the in vivo spatial and temporal organization of DNA replication.

Purpose of the Study:

  • To review the historical progression of understanding DNA replication.
  • To highlight recent technological advancements in studying DNA replication in vivo.
  • To emphasize the integration of new techniques for real-time cellular genome duplication analysis.

Main Methods:

  • Review of historical genetic and in vitro biochemical approaches.
  • Application of genome-wide high-throughput techniques for replication progression analysis.
  • Utilization of novel super-resolution microscopy for single-cell spatial organization studies.

Main Results:

  • Elucidation of the basic mechanism and enzymatic machinery of DNA replication.
  • High-resolution data on genome replication progression using high-throughput techniques.
  • Initial insights into DNA replication organization within intact cells via super-resolution microscopy.

Conclusions:

  • Integrating advanced techniques provides unprecedented views of DNA replication in situ.
  • Real-time filming and dissection of genome replication are becoming feasible.
  • Future research will combine these methods for a comprehensive understanding of cellular DNA duplication.