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Updated: Jun 13, 2026
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Interlaboratory validation of an event-specific real time polymerase chain reaction detection method for genetically
Hiroshi Akiyama1, Kozue Sakata, Frank Spiegelhalter
1National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Abstract:
A real-time polymerase chain reaction (PCR) method specific for genetically modified (GM) maize event DAS59132 (E32) was adapted for qualitative detection of low level presence of E32. The method was validated by a collaborative trial with eight participating Japanese laboratories. Sensitivity was assessed with three different samples of corn flour fortified to 0%, 0.05% and 0.1% (w/w) E32 respectively. In addition, a 0.01% E32 DNA solution was used. The detection limit with DNA solution was estimated to be approximately 0.01%. In conclusion, the results of the study confirmed this real-time PCR method as a reliable tool for qualitative detection of E32 maize.
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