Purification on poly(U)-sepharose 4B of human breast cancer cell line T-47D DNA polymerases

The purification of DNA polymerases (RNA-directed DNA polymerases and DNA-directed DNA polymerases) on poly(U)-Sepharose 4B from a breast tumour cell line (T-47D) is reported. The elution of these enzymes was followed in each fraction by activity measurements with the four primer-templates poly(rA)-oligo(dT)12-18, poly(dA) oligo(dT)12-18, poly(rC)-oligo(dG)12-18 and poly(rCm)-oligo (dG)12-18. The control of the polymerase purification by chromatography was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the pooled active enzymatic fractions.