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Hamster cheek-pouch testing of dental soft polymers
B B Harsanyi1, W C Foong, R E Howell
1Division of Oral Pathology, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Journal of Dental Research
|June 1, 1991
Summary
A new "pouch-in-pouch" technique improves hamster cheek pouch models for dental material irritation testing. This method enhances material retention and reduces surgical artifacts, allowing clearer tissue reaction analysis.
Area of Science:
- Biomaterials Science
- Toxicology
- Oral Pathology
Background:
- The hamster cheek pouch is a recognized model for oral mucosal irritation testing of dental materials.
- Existing techniques face challenges with material retention and surgical artifact interference in histopathological interpretation.
Purpose of the Study:
- To introduce and evaluate a novel "pouch-in-pouch" surgical technique for improved mucous membrane irritation testing.
- To assess the efficacy of the new technique in terms of material retention and histopathological clarity.
Main Methods:
- A "pouch-in-pouch" surgical approach was developed for implanting polymer discs in the hamster cheek pouch.
- Material retention rates were quantified at 14 and 35 days post-implantation.
- Histopathological analysis was performed to differentiate tissue reactions to materials from surgical effects.
Main Results:
- The "pouch-in-pouch" technique demonstrated high retention rates: 97% at 14 days and 87% at 35 days.
- The method effectively distinguished between material-induced tissue responses and surgical artifacts.
- Polymer discs with dibutyltin diacetate (DBTD) or dibutyl phthalate (DBP) plasticizers induced epithelial atypia, papillomas, and dysplasia-like changes.
Conclusions:
- The "pouch-in-pouch" technique offers a superior method for hamster cheek pouch irritation tests, enhancing reliability and interpretability.
- Observed epithelial changes suggest potentially pre-malignant effects of DBTD and DBP, warranting further investigation.