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Updated: Jun 13, 2026

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
Published on: February 17, 2021
Activation of wound healing in aged rats by altering the cellular mitogenic potential
Ji Heon Rhim1, Ik-Soon Jang, Sung Tack Kwon
1Department of Biochemistry and Molecular Biology, Ageing and Apoptosis Research Center, Seoul National University College of Medicine, 28 Yon-gon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Abstract:
We previously showed that lysophosphatidic acid (LPA) and an adenylyl cyclase inhibitor (ACI) stimulate mitogenic activation of senescent human diploid fibroblasts. Because the modulation of cell proliferation may affect wound healing in aged organisms, we studied the effects of LPA and ACI on in vivo skin wound healing in aged Fisher 344 rats. We found that, in aged rats, wound healing improved in animals treated with LPA and/or ACI (relative to untreated controls), as assessed by histological analysis of reepithelialization and immunostaining for proliferating cell nuclear antigen. The age-dependent activation of mitogenic responses by LPA and ACI was confirmed in other cell types. Taken together, our findings suggest that the activation of mitogenic potential in senescent cells by LPA and/or ACI may translate into enhanced in vivo wound healing and tissue regeneration in aged animals.
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