Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Helicobacter pylori catalase.

S L Hazell1, D J Evans, D Y Graham

  • 1School of Microbiology, University of New South Wales, Kensington, Australia.

Journal of General Microbiology
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluoride doped γ-Fe<sub>2</sub>O<sub>3</sub> nanoparticles with increased MRI relaxivity.

Journal of materials chemistry. B·2020
Same author

The transfer of unsterilized material from Mars to Phobos: Laboratory tests, modelling and statistical evaluation.

Life sciences in space research·2019
Same author

Quantification of trace fatty acid methyl esters in diesel fuel by using multidimensional gas chromatography with electron and chemical ionization mass spectrometry.

Journal of separation science·2016
Same author

Structural insights into magnetic clusters grown inside virus capsids.

ACS applied materials & interfaces·2014
Same author

Direct amine-functionalisation of γ-Fe2O3 nanoparticles.

Dalton transactions (Cambridge, England : 2003)·2013
Same author

Mesangial IgA disease with crescent formation during pregnancy: postpartum treatment with immunosuppression.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2013
Same journal

Osmoregulation in Azospirillum brasilense: glycine betaine transport enhances growth and nitrogen fixation under salt stress.

Journal of general microbiology·2012
Same journal

Glutamine metabolism during aerial mycelium growth of Neurospora crassa.

Journal of general microbiology·2011
Same journal

Glutamine requirement for aerial mycelium growth in Neurospora crassa.

Journal of general microbiology·2011
Same journal

Heat-sensitive lysis mutants of Bacillus subtilis 168 blocked at three different stages of peptidoglycan synthesis.

Journal of general microbiology·2010
Same journal

The lecithinase of Clostridium bifermentans and its relation to the alpha-toxin of Clostridium welchii.

Journal of general microbiology·2010
Same journal

The production by certain species of Clostridium of enzymes disintegrating hide powder.

Journal of general microbiology·2010
See all related articles

This study characterizes Helicobacter pylori catalase, an enzyme crucial for bacterial survival in the stomach. Catalase activity is enhanced by serum, and the purified enzyme exhibits specific biochemical properties.

Area of Science:

  • Microbiology
  • Enzymology
  • Biochemistry

Background:

  • Helicobacter pylori is a primary cause of gastroduodenitis.
  • Catalase is potentially vital for H. pylori survival on inflamed gastric mucosa.

Purpose of the Study:

  • To characterize H. pylori catalase.
  • To provide a foundation for in vivo studies of catalase function.

Main Methods:

  • Culturing H. pylori in media with blood, serum, or erythrocytes.
  • Purification and biochemical characterization of catalase.
  • Enzyme kinetics and spectrophotometric analysis.

Main Results:

  • Catalase activity significantly increased with serum in the growth medium.

Related Experiment Videos

  • H. pylori catalase is a tetramer (50,000 subunit Mr), with a pI of 9.0-9.3.
  • The enzyme is stable at 56°C, active across a wide pH range, non-competitively inhibited by azide, and possesses an iron-porphyrin prosthetic group.

    • Conclusions:

    • H. pylori catalase is significantly influenced by host-derived factors like serum.
    • The characterized biochemical properties provide essential data for understanding its role in H. pylori pathogenesis.