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Ultrastructural characterization of human immunodeficiency virus type 1 Gag-containing particles assembled in a
S K Vernon1, S Murthy, J Wilhelm
1Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Philadelphia, Pennsylvania 19101.
The Journal of General Virology
|June 1, 1991
Abstract:
The human immunodeficiency virus type 1 (HIV-1) Gag protein was expressed in A549 cells infected with recombinant adenovirus types 4 and 7, each carrying the HIV-1 gag and pro genes. The Gag protein was assembled into enveloped virus-like particles that budded from plasma and vacuolar membranes. The particles, isolated by precipitation and isopycnic density centrifugation, contained both processed and unprocessed Gag-associated proteins.