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Related Experiment Video

Updated: Jun 13, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

[Evaluation on a fast weight reduction model in vitro].

Songtao Li1, Ying Li, Ying Wen

  • 1Department of Nutrition and Food Hygene, College of Public Health, Harbin Medical University, Harbin 150086, China. lisongtaoklds@126.com

Wei Sheng Yan Jiu = Journal of Hygiene Research
|May 13, 2010
PubMed
Summary
This summary is machine-generated.

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This study developed a rapid in vitro model using rat adipocytes to screen weight-loss drugs. The model effectively identified compounds that stimulate lipolysis, proving useful for drug discovery.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Cell Biology

Context:

  • Obesity is a growing global health concern requiring novel therapeutic strategies.
  • In vitro models are crucial for efficient preclinical drug screening.
  • Developing reliable models for evaluating weight-reducing agents is essential.

Purpose:

  • To establish and validate a rapid in vitro model for screening potential weight-reducing drugs.
  • To assess the efficacy of the established model using known pharmacological agents.

Summary:

  • A novel in vitro obesity model was created using mature Sprague-Dawley rat adipocytes induced by oleic acid.
  • Isoprenaline, genistein, and caffeine were used as positive controls, demonstrating significant lipolysis.
  • Curcumin served as a negative control, showing no significant effect on lipolysis, validating the model's responsiveness.

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Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet
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Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet

Published on: July 31, 2021

Related Experiment Videos

Last Updated: Jun 13, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet
13:09

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet

Published on: July 31, 2021

Impact:

  • This validated model offers a fast and effective platform for the preliminary evaluation of weight-loss drug candidates.
  • Facilitates accelerated discovery of novel anti-obesity therapeutics.
  • Contributes to the development of more efficient drug screening pipelines in metabolic research.