Differentiation potential of STRO-1+ dental pulp stem cells changes during cell passaging
Jinhua Yu1, Huixia He, Chunbo Tang
1Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, China. yuziyi_yjh@hotmail.com
BMC Cell Biology
|May 13, 2010
Summary
Dental pulp stem cells (DPSCs) retain multipotency but alter differentiation potential with passaging. Long-term cultured DPSCs show increased osteogenic gene expression but limited in vivo differentiation capacity.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Dental Research
Background:
- Dental pulp stem cells (DPSCs) are multipotent and can differentiate into various cell types.
- The impact of serial passaging on naive DPSCs' differentiation potential is not fully understood.
Purpose of the Study:
- To investigate the differentiation potential of STRO-1+ DPSCs after long-term serial passaging.
- To analyze changes in gene and protein expression related to osteogenic, odontogenic, and chondrogenic lineages.
Main Methods:
- Isolation of STRO-1+ DPSCs from human/rat dental pulps using magnetic activated cell sorting.
- Culture and serial passaging of DPSCs in conventional media.
- Assessment of proliferation, gene/protein expression, and in vitro/in vivo differentiation capacity.
Main Results:
- Proliferation of human DPSCs decreased with passaging.
- STRO-1+ DPSCs at the 9th passage (DPSC-P9) showed upregulated osteoblast, odontoblast, and chondrocyte-specific gene/protein expression compared to the 1st passage (DPSC-P1).
- In vivo, DPSC-P1 cells formed dentin, bone, and cartilage, while DPSC-P9 cells formed only bone.
Conclusions:
- STRO-1+ DPSCs comprise subpopulations with spontaneous differentiation capabilities.
- Cell passaging alters the differentiation capacity of DPSCs.
- In vivo, late-passage DPSCs (9th passage) exhibit restricted differentiation potential, primarily to the osteoblast lineage.
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