Related Experiment Video
Updated: Jun 13, 2026
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
Published on: August 4, 2016
Immunohistochemical study of cytokeratin expression in nevus sebaceous
Ichiro Kurokawa1, Keisuke Nishimura, Kei-ichi Yamanaka
1Department of Dermatology, Mie University Graduate School of Medicine, Mie, Japan. kuroichi@clin.medic.mie-u.ac.jp
Background:
The histogenesis of nevus sebaceous (NS) is unclear.
Methods:
To elucidate the histogenesis of NS, cytokeratin (CK) profiles were examined immunohistochemically using 10 anti-keratin antibodies in the three stages of NS.
Results:
In the first stage, stratified differentiated keratins (CK1 and 10) were reduced, and basal keratin (CK14) was increased in the epidermis and primitive follicular structure (PFS). In the second stage, in addition to reduced CK1 and CK10 expressions and increased CK14 expression, CK17 expression was strongly expressed in the sebaceous ducts in proportion to the development of sebaceous gland. In the third stage, CK14, CK17 and CK19 were expressed in secondary tumors. CK16 was not detected throughout all stages of NS.
Conclusion:
These results suggest that NS is not hyperproliferative but involves hamartomatous differentiation with undifferentiated keratins.
