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Related Experiment Video

Updated: Jun 13, 2026

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Published on: November 5, 2020

Methods in tubulin proteomics.

Leah M Miller¹, Hui Xiao, Berta Burd

¹Laboratory of Macromolecular Analysis and Proteomics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Methods in Cell Biology

|May 15, 2010

Summary

This summary is machine-generated.

New proteomic methods enhance the study of microtubule dynamics and drug interactions. These techniques identify tubulin isotypes and posttranslational modifications, aiding in targeted drug design for microtubule-related diseases.

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Last Updated: Jun 13, 2026

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

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Optimizing Tubulin Yield from Porcine Brain Tissue

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Published on: October 11, 2024

Area of Science:

Biochemistry
Cell Biology
Proteomics

Background:

Microtubule dynamics are crucial for cellular functions.
Understanding drug interactions with microtubules requires advanced analytical methods.
Tubulin isotype diversity and posttranslational modifications (PTMs) influence microtubule behavior.

Purpose of the Study:

To present protocols for analyzing tubulin isotypes and PTMs.
To identify tubulin regions affected by microtubule-stabilizing agents.
To advance the understanding of tubulin proteomics for drug development.

Main Methods:

Mass spectrometry (MS)-based proteomics.
Chromatographic and electrophoretic separation of tubulin.
Analysis of tubulin from cultured cells and tissues.

Main Results:

Successful characterization of tubulin isotypes and PTMs.
Identification of specific tubulin regions interacting with drugs.
Development of protocols for tubulin analysis.

Conclusions:

Proteomic methodologies are essential for studying microtubule dynamics.
These methods facilitate the identification of tubulin isotype-specific functions.
The developed techniques aid in designing targeted drugs for microtubule-related therapies.