Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.

Plant Cell Wall

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.Collenchyma and sclerenchyma cells, on the other hand, mainly occur in the outer layers of a plant's stems and leaves. These cells provide the plant with strength and support by either partially thickening their primary cell wall (i.e., collenchyma), or depositing a...

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

Microbe-Plant Interactions

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Horizontal acquisition of nicotine catabolism gene cluster enhances Arthrobacter fitness within tobacco root microbiota.

Microbiome·2026

Same author

A copper-dependent redox-based hydrogen peroxide perception in plants.

Nature communications·2026

Same author

Whole-genome sequencing of diverse 351 cultured prokaryotes including as-yet-unsequenced type strains.

Genome research·2026

Same author

Plant cell surface receptors.

The Plant journal : for cell and molecular biology·2026

Same author

WIND1 controls cell fate transition through coordinately integrating histone acetylation and deacetylation-mediated transcriptional reprogramming during somatic embryogenesis.

Molecular plant·2026

Same author

Dihydroxyhexanoic acid biosynthesis controls turgor in pathogenic fungi.

Science (New York, N.Y.)·2026

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Ubiquitination in plant immunity.

Marco Trujillo¹, Ken Shirasu

¹Julius-von-Sachs Institute, Department of Pharmaceutical Biology, University of Würzburg, Julius-von-Sachs Platz 2, Würzburg, Germany.

Current Opinion in Plant Biology

|May 18, 2010

Summary

The plant immune system relies on ubiquitination (UBS) and proteasome pathways. Pathogens target these systems, highlighting their crucial role in plant immunity and defense.

Area of Science:

Plant immunity
Molecular biology
Biochemistry

Background:

Plant immune responses involve complex, coordinated processes triggered by pathogen detection.
The ubiquitination system (UBS) and 26S proteasome are critical regulators of key immune processes.
E3 ligases, as specificity determinants in ubiquitination, are central to these regulatory mechanisms.

Purpose of the Study:

To review the role of the ubiquitination system (UBS) and 26S proteasome in plant immunity.
To highlight the function of E3 ligases in regulating plant defense responses.
To emphasize the importance of the UBS in plant immunity by examining pathogen targeting.

Main Methods:

Literature review and synthesis of existing research on plant ubiquitination and immunity.

More Related Videos

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Related Experiment Videos

Last Updated: Jun 13, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Analysis of the roles of single-unit and multisubunit E3 ligases in plant defense.

Examination of evidence detailing pathogen virulence effector interactions with the plant UBS.

Main Results:

The UBS and 26S proteasome regulate essential immune processes like oxidative burst, hormone signaling, gene induction, and programmed cell death.
Single-unit E3 ligases, rapidly induced by pathogens, act as both positive and negative regulators of immunity.
Multisubunit E3 ligases are primarily implicated in plant hormone signaling pathways.
Pathogen virulence effectors frequently target the plant UBS, underscoring its significance in immunity.

Conclusions:

The ubiquitination system is a pivotal component of plant immunity, essential for regulating diverse defense mechanisms.
E3 ligases play multifaceted roles in plant immunity, with distinct functions for single-unit and multisubunit types.
Pathogen manipulation of the UBS highlights its critical role and represents a key battleground in plant-pathogen interactions.