Related Experiment Video
Updated: Jun 13, 2026
Chronic Sleep Deprivation in Mouse Pups by Means of Gentle Handling
Published on: October 11, 2018
[Effects of CRF receptor antagonist on rem sleep in neonatal rat]
Xue-dong Liu1, Hong-kun Fan, Gui-hong Zhang
1Department of Physiology, Medical School in Zhengzhou University, Zhengzhou 450052, China.
Objective:
To observe the role of NB127914, a CRF R1 receptor antagonist, in the regulation of neonatal sleep/wake cycle.
Methods:
Rat pups were surgically implanted with electrodes at postnatal day(PN) 13. At PN 14, 6 hours polysomnographic recording data were continuously collected before and after administration of various doses of NBI 27914, atropine and the same amount of saline.
Results:
Compared with baseline, rapid eye movement (REM) sleep was significantly reduced and was replaced primarily by non-REM (NREM) sleep in all groups treated with NBI, but not with dimethyl sulfoxide/saline. Atropine suppressed REM sleep significantly and increased wakefulness simultaneously.
Conclusion:
Blockage of corticotropin-releasing factor (CRF) R1 receptors deprives neonatal rat REM sleep.
