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Updated: Jun 13, 2026
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
Published on: October 19, 2013
[Primary cell culture and identification methods of rat pulmonary arterial smooth muscle cells]
Guo-qing Qian1, Liang-xing Wang, Chan Cheng
1Department of Respiratory, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
Objective:
Set up a method to isolate and identify the small pulmonary arterial smooth muscle cells (PASMCs) in vitro.
Methods:
In sterile conditions, separated the male SD rat pulmonary artery, digested by collagenase I and cultured primary PASMCs. Measured cell viability; observed by phase contrast microscope; identified by immunocytochemistry and immunofluorescence staining as a label for smooth muscle alpha-actin.
Results:
PASMCs were identified by morphology and immunocytochemistry, immunofluorescence staining, with the cell viability is over 96.5%. The primary culture could be subcultured after 4-7 days and successfully passaged without change in morphology and growth characteristic.
Conclusion:
This technique has advantage of the method is simple, short cultivate, good reproducibility, the primary cultured PASMCs quantity and the rapid growth.
