Related Experiment Video
Updated: Jun 13, 2026
10:34
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Massive parallel sequencing in animal genetics: wherefroms and wheretos
1Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. miguel.perez@uab.es
Animal Genetics
|May 19, 2010
Summary
Next-generation sequencing (NGS) provides complete genomes and variant catalogs for non-mainstream species. RNA-seq aids gene annotation, enabling advanced applications like whole-genome association studies and breeding programs.
Area of Science:
- Genomics
- Animal Genetics
- Bioinformatics
Background:
- Next-generation sequencing (NGS) has transformed biological research.
- Genomic studies in non-mainstream species are now more feasible.
- RNA-seq offers a powerful method for gene annotation.
Purpose of the Study:
- To highlight the impact and applications of NGS in genomics.
- To discuss experimental design considerations for NGS.
- To encourage adoption of NGS technologies in animal genetics.
Main Methods:
- Whole genome sequencing for comprehensive variant cataloging.
- RNA-sequencing (RNA-seq) for gene annotation.
- Discussion of experimental designs like pooling and reduced representation libraries.
Main Results:
- NGS enables complete genome sequencing and variant cataloging for diverse species.
- RNA-seq effectively annotates genes beyond computational predictions.
- Sequencing offers advantages over genotyping, including copy number variant identification.
Conclusions:
- NGS technologies are revolutionizing biological and animal genetics research.
- Future applications include whole-genome association studies and improved breeding programs.
- Despite bioinformatics challenges, NGS adoption is encouraged for its transformative potential.
Related Concept Videos
02:54Evolutionary Relationships through Genome Comparisons
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
02:02Genomics
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
02:51Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
01:57Sanger Sequencing
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
01:05Maxam-Gilbert Sequencing
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...