Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclic AMP and cyclic GMP in rabbit blastocysts.

S K Dey, F Kimura, A Mukherjee

    Journal of Reproduction and Fertility
    |March 1, 1978
    PubMed
    Summary

    Nucleotide concentrations increased significantly in Day-6 compared to Day-5 blastocysts. The cyclic adenosine monophosphate (cAMP) to cyclic guanosine monophosphate (cGMP) ratio also shifted, indicating developmental changes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Search for Gluino-Mediated Bottom Squark Production in pp[over ] Collisions at sqrt[s]=1.96 TeV.

    Physical review letters·2009
    Same author

    Search for exclusive Z-boson production and observation of high-mass pp[over ]-->pgammagammap[over ]-->pl;{+}l;{-}p[over ] events in pp[over ] collisions at sqrt[s]=1.96 TeV.

    Physical review letters·2009
    Same author

    First measurement of the tt[over ] differential cross section dsigma/dM_{tt[over ]} in pp[over ] collisions at sqrt[s]=1.96 TeV.

    Physical review letters·2009
    Same author

    Measurement of the k(T) distribution of particles in jets produced in pp collisions at sqrt(s)=1.96 TeV.

    Physical review letters·2009
    Same author

    Genotoxicity of silver nanoparticles in Allium cepa.

    The Science of the total environment·2009
    Same author

    Search for top-quark production via flavor-changing neutral currents in W+1 jet events at CDF.

    Physical review letters·2009

    Area of Science:

    • Reproductive Biology
    • Developmental Biology
    • Molecular Biology

    Background:

    • Blastocyst development is a critical stage in early mammalian embryogenesis.
    • Intracellular nucleotide signaling plays a vital role in embryonic development.

    Purpose of the Study:

    • To investigate the changes in cyclic nucleotide concentrations during blastocyst development.
    • To determine the ratio of cyclic adenosine monophosphate (cAMP) to cyclic guanosine monophosphate (cGMP) in Day-5 and Day-6 blastocysts.

    Main Methods:

    • Quantification of cAMP and cGMP levels in Day-5 and Day-6 mouse blastocysts.
    • Comparative analysis of nucleotide concentrations and their ratios between developmental stages.

    Main Results:

    • Significant increases in both cAMP and cGMP concentrations were observed from Day-5 to Day-6 blastocysts.
    • The ratio of cAMP to cGMP significantly increased from 0.5 in Day-5 to 1.5 in Day-6 blastocysts.

    Conclusions:

    • Blastocyst development is associated with significant alterations in intracellular cyclic nucleotide levels.
    • The shift in the cAMP/cGMP ratio suggests a role for these signaling molecules in blastocyst differentiation and maturation.

    Related Experiment Videos