Related Experiment Video
Updated: Jun 13, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
Published on: September 21, 2011
Modulation of steroid hormone receptor activity
Vladimir Stanisić1, David M Lonard, Bert W O'Malley
1Department of Molecular and Cellular Biology, Baylor College of Medicine, Texas, United States of America.
Steroid hormones (SHs) regulate key bodily functions via steroid hormone receptors (SHRs). Cells control SHR activity through expression levels and post-translational modifications to ensure proper physiological responses.
Area of Science:
- Endocrinology
- Molecular Biology
- Genetics
Background:
- Classical steroid hormones (SHs) like estrogens and androgens are vital for reproduction, metabolism, and cancer.
- SHs exert effects through steroid hormone receptors (SHRs), which are nuclear receptors (NRs) that regulate gene transcription.
- SHRs mediate steroid hormone signaling, making their regulation crucial for physiological responses.
Purpose of the Study:
- To examine the regulatory mechanisms controlling steroid hormone receptor (SHR) expression and activity.
- To assess the functional impact of these regulatory mechanisms on SHR activity.
Main Methods:
- Review of cellular mechanisms regulating SHR protein concentration.
- Analysis of post-translational modifications affecting SHR activity.
- Examination of coactivator protein complex interactions with SHRs.
Main Results:
- Cells precisely control SHR protein levels to modulate steroid hormone signaling.
- Post-translational modifications and coactivator interactions fine-tune SHR activity.
- These regulatory layers ensure appropriate cellular responses to steroid hormones.
Conclusions:
- Cellular regulation of SHR expression and activity is essential for normal physiology.
- Understanding these mechanisms provides insight into steroid hormone-related diseases.
- Targeting SHR regulation offers potential therapeutic strategies.
More Related Videos
10:51Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
09:07Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
Related Concept Videos
Transducer Mechanism: Nuclear Receptors
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Target Cell Response to Hormones
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Types of Receptors: Internal Receptors
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
Internal Receptors
Intracellular Hormone Receptors
The Two-State Receptor Model
The binding affinity of a drug determines its interaction with one...