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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: Jun 13, 2026

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Live cell-based sensor cells.

Akiyoshi Taniguchi¹

¹Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki, Japan. taniguchi.akiyoshi@nims.go.jp

|May 19, 2010

Summary

This summary is machine-generated.

Researchers developed live cell-based biosensors for rapid and sensitive cytotoxicity detection. This novel sensorchip, using microfluidic channels, offers high-throughput analysis for various applications.

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Last Updated: Jun 13, 2026

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

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Published on: December 16, 2017

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Published on: October 28, 2013

Area of Science:

Biotechnology
Cell-based biosensors
Microfluidics

Background:

Live cell biosensors offer high specificity and sensitivity for target detection.
Cytotoxicity detection is crucial for biomaterials, nanotoxicology, environmental assessment, and drug screening.

Purpose of the Study:

To establish rapid, sensitive, and high-throughput cytotoxic sensor cells.
To develop a live cell-based sensorchip utilizing microfluidic channels for quantitative cytotoxicity detection.

Main Methods:

Transfection of sensor cells with GFP plasmids under HSP70B and BTG2 promoters.
Fabrication of a microfluidic channel-based sensorchip.
Monitoring GFP fluorescence in sensor cells in response to CdCl(2) exposure.

Main Results:

Sensor cells exhibited increased GFP fluorescence in a dose-dependent manner with CdCl(2).
The microfluidic system enabled quick and sensitive detection of cytotoxic reagents.
The system demonstrated potential for high-throughput analysis.

Conclusions:

The developed live cell-based sensorchip is a rapid, sensitive, and high-throughput tool for cytotoxicity detection.
This technology provides a foundation for micro total analysis systems (mu-TAS) in diverse fields.
Potential applications include drug screening, environmental monitoring, and nanotoxicology.