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Related Experiment Video

Updated: Jun 13, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Oxygen measurements via phosphorescence.

Sami Shaban¹, Farida Marzouqi, Aysha Al Mansouri

¹Department of Medical Education, United Arab Emirates University, Faculty of Medicine and Health Sciences, Al Ain, United Arab Emirates. sami.shaban@uaeu.ac.ae

Computer Methods and Programs in Biomedicine

|May 19, 2010

Summary

This study introduces a novel method for measuring dissolved oxygen using a palladium phosphor probe. The developed software enables accurate, real-time monitoring of oxygen consumption in biological samples.

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Area of Science:

Analytical Chemistry
Biochemistry
Biophysics

Background:

Accurate dissolved oxygen (O(2)) measurements are crucial for various chemical and biological applications.
Existing methods may lack the precision or customization required for dynamic biological systems.
Phosphorescence quenching by oxygen offers a sensitive detection principle.

Purpose of the Study:

To develop and validate a customized system for accurate, time-resolved dissolved oxygen measurements.
To enable reliable monitoring of cellular oxygen consumption over extended periods.

Main Methods:

Utilized a palladium (II) complex of meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin (Pd phosphor) as an oxygen probe.
Employed a phosphorescence detection system with a 625nm excitation source and 800nm emission detection.
Digitized phosphorescence pulses using a PCI-DAS 4020/12 I/O Board (1-20MHz).
Developed custom software (Microsoft Visual Basic 6, Microsoft Access Database 2007) for data acquisition (0.1-4MHz), storage, and analysis.
Calculated O(2) concentration by fitting phosphorescence decay to an exponential function.

Main Results:

A customized software program was successfully developed for precise control of data acquisition, storage, and analysis.
The system allowed for reliable measurements of cellular O(2) consumption over several hours.
A relational database design facilitated efficient storage of experimental data, including phosphorescence decay rates.

Conclusions:

The developed phosphorescence-based method provides a reliable and customizable approach for dissolved oxygen monitoring.
This technique is suitable for long-term studies of oxygen dynamics in biological samples.
The integrated software and hardware system enhances the accuracy and efficiency of oxygen measurements.