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Updated: Jun 13, 2026
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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
Published on: June 30, 2017
Cord lining progenitor cells: potential in vitro adipogenesis model
H H Cheong1, J Masilamani, T T Phan
1Department of Pharmacy, National University of Singapore, Singapore, Singapore.
International Journal of Obesity (2005)
|May 19, 2010
Summary
Umbilical cord lining cells differentiate into adipocyte-like cells (ALCs). Glucose and insulin levels influence ALC secretions, suggesting potential for studying obesity and metabolic disorders.
Area of Science:
- Cell Biology
- Metabolic Research
Background:
- Adipogenesis is crucial for energy homeostasis.
- Understanding progenitor cell differentiation is key to metabolic research.
Purpose of the Study:
- Investigate glucose and insulin effects on umbilical cord lining progenitor cell differentiation into adipocyte-like cells (ALCs).
Main Methods:
- Isolated cord lining mesenchymal cells (CLMCs) and induced differentiation.
- Confirmed lipid droplet formation using Oil Red O staining.
- Analyzed gene expression (adipsin, PPARγ) and protein secretion (leptin, adiponectin).
Main Results:
- CLMCs differentiated into ALCs with upregulated adipsin and PPARγ.
- ALCs in low-glucose medium secreted leptin and adiponectin; high-glucose medium led to leptin secretion only.
- Insulin affected leptin secretion but not adiponectin.
Conclusions:
- CLMCs can differentiate into ALCs under varying culture conditions.
- These ALCs serve as a viable in vitro model for adipogenesis and obesity studies.
- Potential application in drug discovery for metabolic disorders.
